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Fresh transverse section of a sabre root. ) Whole mount of a one week old pam pam root. ) Fresh transverse section of a one week old pam pom root. ) Whole mount of a three week pom pam root. ) Fresh transverse section of a three week old pom pom root. Bar = 50 IJm. Abbreviations: C, cortex; E, epidermis; En, endodermis; S, stele. 35 The cross-sectional areas of the epidermal cells in pam pam and cobra were approximately the same and 15 times larger than wild type (Fig. 2A). Also the crosssectional areas of the endodermis and the stele tissue were very similar for these two mutants.
We took this as an indication that root and hypocotyl arise from two embryonic tiers in which at least epidermal cells, after the separation of these tiers, have very limited potential to switch root or hypocotyl fate. The next-largest root sectors found with similar boundaries fall in two classes: interzone sectors in which the lower sector boundary coincides approximately with the region in the root where the interzone-specific second cortex layer disappears; and root sectors originating from the meristem and having their upper sector boundary in the same region.
Some cells are temporarily arrested in Go or have a low probability of passing from A state). but this is based on comparisons of DTN using different methods of analysis which estimate cell cycle parameters that pertain to different fractions of the QC population as a whole (Clowes 1971). Unfortunately, no cytological markers are known for Go cells of plant meristems as are claimed to exist in animal cell populations (Cohen et al. 1981). However, long-term labelling of the QC with 3H-thymidine does reveal a group of cells at the pole of the stele having a longer pre-DNA-replication phase than cells of the cortical portion of the QC (Barlow 1978).